| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ristuccia, Eden L (11)
|
NJPPST |
41.76 |
40.23 |
-1.53 |
| 2 |
Dronadula, Pranavi (12)
|
NJPAA |
44.05 |
44.61 |
0.56 |
| 3 |
Xu, Emma A (12)
|
NJGSCY |
44.07 |
44.71 |
0.64 |
| 4 |
Henriques, Samantha A (11)
|
NJBAC |
44.79 |
45.61 |
0.82 |
| 5 |
Song, Annabelle (11)
|
NJPAA |
47.95 |
46.54 |
-1.41 |
| 6 |
D'Agosta-Serravallo, Lola Q (12)
|
NJBAC |
55.65 |
46.65 |
-9.00 |
| 7 |
Cheng, Xiao-Yi A (12)
|
NJPAA |
44.97 |
47.25 |
2.28 |
| 8 |
Richardson, Avery E (12)
|
NJPAA |
50.23 |
47.33 |
-2.90 |
| 9 |
Mishra, Manvi (12)
|
NJPAA |
50.41 |
49.89 |
-0.52 |
| 10 |
Sahu, Aanvi (11)
|
NJGSCY |
48.84 |
52.62 |
3.78 |
|
Vattappallil, Isabella M (11)
|
NJPAA |
40.43 |
NS |
- |